› 5k5s

X-ray diffraction
2.6Å resolution

Crystal structure of the active form of human calcium-sensing receptor extracellular domain

Released:
DOI: 10.2210/pdb5k5s/pdb
PDB entry 5k5s coloured by chain, front view.
PDB entry 5k5s coloured by chain, side view.
PDB entry 5k5s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism of ligand activation in human calcium-sensing receptor.
Geng Y, Mosyak L, Kurinov I, Zuo H, Sturchler E, Cheng TC, Subramanyam P, Brown AP, Brennan SC, Mun HC, Bush M, Chen Y, Nguyen TX, Cao B, Chang DD, Quick M, Conigrave AD, Colecraft HM, McDonald P, Fan QR
Elife 5 (2016)
PMID: 27434672

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular calcium-sensing receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 615 amino acids
Theoretical weight: 69.24 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41180 (Residues: 20-607; Coverage: 56%)
Gene names: CASR, GPRC2A, PCAR1
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand TRP 2 x TRP
Ligand PO4 4 x PO4
Ligand CA 8 x CA
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 107.66Å b: 127.45Å c: 146.77Å
α: 90° β: 108.72° γ: 90°
R-values:
R R work R free
0.212 0.211 0.222
Expression system: Spodoptera frugiperda

Quick links

Citations

40 review citations

G Protein-Coupled Receptors in Taste Physiology and Pharmacology.
Ahmad et al. (2020)
39 more

25 mentions without citation

Structural biology of GABAB receptor.
Frangaj et al. (2018)
24 more