› 5k5t

X-ray diffraction
3.1Å resolution

Crystal structure of the inactive form of human calcium-sensing receptor extracellular domain

Released:
DOI: 10.2210/pdb5k5t/pdb
PDB entry 5k5t coloured by chain, front view.
PDB entry 5k5t coloured by chain, side view.
PDB entry 5k5t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism of ligand activation in human calcium-sensing receptor.
Geng Y, Mosyak L, Kurinov I, Zuo H, Sturchler E, Cheng TC, Subramanyam P, Brown AP, Brennan SC, Mun HC, Bush M, Chen Y, Nguyen TX, Cao B, Chang DD, Quick M, Conigrave AD, Colecraft HM, McDonald P, Fan QR
Elife 5 (2016)
PMID: 27434672

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-154256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular calcium-sensing receptor Chain: A
Molecule details ›
Chain: A
Length: 615 amino acids
Theoretical weight: 69.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41180 (Residues: 20-607; Coverage: 56%)
Gene names: CASR, GPRC2A, PCAR1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand SO4 3 x SO4
Ligand NAG 7 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: F222
Unit cell:
a: 126.33Å b: 150.15Å c: 214.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.239
Expression system: Spodoptera frugiperda

Quick links

Citations

41 review citations

G Protein-Coupled Receptors in Taste Physiology and Pharmacology.
Ahmad et al. (2020)
40 more

17 mentions without citation

Structural biology of GABAB receptor.
Frangaj et al. (2018)
16 more