› 5k9i

X-ray diffraction
2.5Å resolution

Crystal structure of c-SRC in complex with a covalent lysine probe

Released:
DOI: 10.2210/pdb5k9i/pdb
PDB entry 5k9i coloured by chain, front view.
PDB entry 5k9i coloured by chain, side view.
PDB entry 5k9i coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Broad-Spectrum Kinase Profiling in Live Cells with Lysine-Targeted Sulfonyl Fluoride Probes.
Zhao Q, Ouyang X, Wan X, Gajiwala KS, Kath JC, Jones LH, Burlingame AL, Taunton J
J Am Chem Soc 139 680-685 (2017)
PMID: 28051857

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.73 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00523 (Residues: 251-533; Coverage: 53%)
Gene name: SRC
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand O44 2 x O44
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P1
Unit cell:
a: 42.012Å b: 63.306Å c: 73.77Å
α: 78.85° β: 88.75° γ: 89.93°
R-values:
R R work R free
0.193 0.191 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Kinase inhibitors: the road ahead.
Ferguson et al. (2018)
24 more

3 mentions without citation

Structure-based design and analysis of SuFEx chemical probes.
Jones et al. (2020)
2 more