› 5kdo

X-ray diffraction
1.9Å resolution

Heterotrimeric complex of the 4 alanine insertion variant of the Gi alpha1 subunit and the Gbeta1-Ggamma1

Released:
DOI: 10.2210/pdb5kdo/pdb
PDB entry 5kdo coloured by chain, front view.
PDB entry 5kdo coloured by chain, side view.
PDB entry 5kdo coloured by chain, top view.
Source organisms:
Primary publication:
A Conserved Hydrophobic Core in Gαi1 Regulates G Protein Activation and Release from Activated Receptor.
Kaya AI, Lokits AD, Gilbert JA, Iverson TM, Meiler J, Hamm HE
J Biol Chem 291 19674-86 (2016)
PMID: 27462082

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136058 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 40.68 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 1-333, 334-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Bos taurus
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(T) subunit gamma-T1 Chain: G
Molecule details ›
Chain: G
Length: 74 amino acids
Theoretical weight: 8.56 KDa
Source organism: Bos taurus
Expression system: Bos taurus
UniProt:
  • Canonical: P02698 (Residues: 1-74; Coverage: 100%)
Gene name: GNGT1
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P43
Unit cell:
a: 84.648Å b: 84.648Å c: 130.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.208
Expression systems:
  • Escherichia coli
  • Bos taurus

Quick links

Citations

4 review citations

Structure and dynamics of GPCR signaling complexes.
Hilger et al. (2018)
3 more

11 mentions without citation

Structure and metal-binding properties of lipopolysaccharides from heptoseless mutants of Escherichia coli studied by 13C and 31P nuclear magnetic resonance.
Strain et al. (1983)
10 more