› 5kjx

X-ray diffraction
1.9Å resolution

Co-crystal Structure of PKA RI alpha CNB-B domain with cAMP

Released:
DOI: 10.2210/pdb5kjx/pdb
PDB entry 5kjx coloured by chain, front view.
PDB entry 5kjx coloured by chain, side view.
PDB entry 5kjx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mutations of PKA cyclic nucleotide-binding domains reveal novel aspects of cyclic nucleotide selectivity.
Lorenz R, Moon EW, Kim JJ, Schmidt SH, Sankaran B, Pavlidis IV, Kim C, Herberg FW
Biochem J 474 2389-2403 (2017)
PMID: 28583991

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase type I-alpha regulatory subunit Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10644 (Residues: 234-381; Coverage: 39%)
Gene names: PKR1, PRKAR1, PRKAR1A, TSE1
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CMP 1 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 37.897Å b: 52.207Å c: 74.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.226
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Popeye Domain Containing Genes and Their Function as cAMP Effector Proteins in Striated Muscle.
Brand T. (2018)
2 more

4 mentions without citation

Mutations of PKA cyclic nucleotide-binding domains reveal novel aspects of cyclic nucleotide selectivity.
Lorenz et al. (2017)
3 more