› 5kjz

X-ray diffraction
1.35Å resolution

Co-crystal structure of PKA RI alpha CNB-B mutant (G316R/A336T) with cGMP

Released:
DOI: 10.2210/pdb5kjz/pdb
PDB entry 5kjz coloured by chain, front view.
PDB entry 5kjz coloured by chain, side view.
PDB entry 5kjz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mutations of PKA cyclic nucleotide-binding domains reveal novel aspects of cyclic nucleotide selectivity.
Lorenz R, Moon EW, Kim JJ, Schmidt SH, Sankaran B, Pavlidis IV, Kim C, Herberg FW
Biochem J 474 2389-2403 (2017)
PMID: 28583991

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase type I-alpha regulatory subunit Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 17.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10644 (Residues: 234-381; Coverage: 39%)
Gene names: PKR1, PRKAR1, PRKAR1A, TSE1
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand PCG 1 x PCG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 29.461Å b: 67.78Å c: 77.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.182
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Popeye Domain Containing Genes and Their Function as cAMP Effector Proteins in Striated Muscle.
Brand T. (2018)
2 more

2 mentions without citation

Peptide Correlation Analysis (PeCorA) Reveals Differential Proteoform Regulation.
Dermit et al. (2021)
1 more