› 5ko1

X-ray diffraction
2.16Å resolution

Pseudokinase Domain of MLKL bound to Compound 4.

Released:
DOI: 10.2210/pdb5ko1/pdb
PDB entry 5ko1 coloured by chain, front view.
PDB entry 5ko1 coloured by chain, side view.
PDB entry 5ko1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
ATP-Competitive MLKL Binders Have No Functional Impact on Necroptosis.
Ma B, Marcotte D, Paramasivam M, Michelsen K, Wang T, Bertolotti-Ciarlet A, Jones JH, Moree B, Butko M, Salafsky J, Sun X, McKee T, Silvian LF
PLoS One 11 e0165983 (2016)
PMID: 27832137

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mixed lineage kinase domain-like protein Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NB16 (Residues: 191-471; Coverage: 60%)
Gene name: MLKL
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 6UY 1 x 6UY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 71.4Å b: 74.88Å c: 127.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.226
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Molecular Insights into the Mechanism of Necroptosis: The Necrosome As a Potential Therapeutic Target.
Chen et al. (2019)
4 more

3 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
2 more