› 5kwn

X-ray diffraction
1.42Å resolution

The WD domain ofArabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from HY5

Released:
DOI: 10.2210/pdb5kwn/pdb
PDB entry 5kwn coloured by chain, front view.
PDB entry 5kwn coloured by chain, side view.
PDB entry 5kwn coloured by chain, top view.
Source organisms:
Entry authors: Uljon S, Blacklow S

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127678 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase COP1 Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 38.17 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P43254 (Residues: 349-675; Coverage: 48%)
Gene names: At2g32950, COP1, T21L14.11
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Transcription factor HY5 Chain: U
Molecule details ›
Chain: U
Length: 19 amino acids
Theoretical weight: 2.09 KDa
Source organism: Arabidopsis
Expression system: Not provided
UniProt:
  • Canonical: O24646 (Residues: 34-52; Coverage: 11%)
Gene names: At5g11260, BZIP56, F2I11_150, HY5

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Unit cell:
a: 47.99Å b: 55.176Å c: 131.618Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 0.174
Expression systems:
  • Trichoplusia ni
  • Not provided

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