5kwy

X-ray diffraction
2.4Å resolution

Structure of human NPC1 middle lumenal domain bound to NPC2

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-127301 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NPC intracellular cholesterol transporter 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Bacteria
UniProt:
  • Canonical: O15118 (Residues: 374-620; Coverage: 20%)
Gene name: NPC1
NPC intracellular cholesterol transporter 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 133 amino acids
Theoretical weight: 14.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P61916 (Residues: 20-151; Coverage: 100%)
Gene names: HE1, NPC2
Sequence domains: ML domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2221
Unit cell:
a: 98.956Å b: 109.686Å c: 154.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.249
Expression systems:
  • Bacteria
  • Homo sapiens