› 5l0q

X-ray diffraction
2.76Å resolution

Crystal structure of the complex between ADAM10 D+C domain and a conformation specific mAb 8C7.

Released:
DOI: 10.2210/pdb5l0q/pdb
PDB entry 5l0q coloured by chain, front view.
PDB entry 5l0q coloured by chain, side view.
PDB entry 5l0q coloured by chain, top view.
Source organisms:
Primary publication:
An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth.
Atapattu L, Saha N, Chheang C, Eissman MF, Xu K, Vail ME, Hii L, Llerena C, Liu Z, Horvay K, Abud HE, Kusebauch U, Moritz RL, Ding BS, Cao Z, Rafii S, Ernst M, Scott AM, Nikolov DB, Lackmann M, Janes PW
J Exp Med 213 1741-57 (2016)
PMID: 27503072

Function and Biology Details

Reaction catalysed: 
Endopeptidase of broad specificity.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231359 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Disintegrin and metalloproteinase domain-containing protein 10 Chains: A, D
Molecule details ›
Chains: A, D
Length: 203 amino acids
Theoretical weight: 21.78 KDa
Source organism: Bos taurus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q10741 (Residues: 455-646; Coverage: 26%)
Gene names: ADAM10, MADM
Sequence domains:
mAb 8C7 light chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 214 amino acids
Theoretical weight: 23.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
mAb 8C7 heavy chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 222 amino acids
Theoretical weight: 23.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand NAG 4 x NAG
Ligand SO4 13 x SO4
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 53.326Å b: 141.679Å c: 268.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.251
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Targeting ADAM10 in Cancer and Autoimmunity.
Smith et al. (2020)
7 more

5 mentions without citation

Domain integration of ADAM family proteins: Emerging themes from structural studies.
Seegar et al. (2019)
4 more