› 5l1z

X-ray diffraction
5.9Å resolution

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130023 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Cyclin-dependent kinase 9 Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 38.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50750 (Residues: 1-330; Coverage: 89%)
Gene names: CDC2L4, CDK9, TAK
Sequence domains: Protein kinase domain
Cyclin-T1 Chain: B
Molecule details ›
Chain: B
Length: 264 amino acids
Theoretical weight: 30.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60563 (Residues: 1-264; Coverage: 36%)
Gene name: CCNT1
Sequence domains:
AF4/FMR2 family member 4 Chain: C
Molecule details ›
Chain: C
Length: 36 amino acids
Theoretical weight: 4.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UHB7 (Residues: 32-67; Coverage: 3%)
Gene names: AF5Q31, AFF4, HSPC092, MCEF
Protein Tat Chain: D
Molecule details ›
Chain: D
Length: 58 amino acids
Theoretical weight: 6.78 KDa
Source organism: Human immunodeficiency virus type 1 BH10
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P69697 (Residues: 1-57; Coverage: 66%)
Gene name: tat
Sequence domains: Transactivating regulatory protein (Tat)
RNA (5'-R(P*AP*GP*AP*UP*CP*UP*GP*AP*GP*CP*CP*UP*GP*GP*GP*AP*GP*CP*UP*CP*UP*CP*U)-3') Chain: N
Molecule details ›
Chain: N
Length: 23 nucleotides
Theoretical weight: 7.36 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3221
Unit cell:
a: 146.87Å b: 146.87Å c: 103.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.313
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

7 review citations

The Molecular Basis for Human Immunodeficiency Virus Latency.
Mbonye et al. (2017)
6 more

7 mentions without citation

Structural insights into the functional diversity of the CDK-cyclin family.
Wood et al. (2018)
6 more