› 5l4k

Electron Microscopy
4.5Å resolution

The human 26S proteasome lid

Released:
DOI: 10.2210/pdb5l4k/pdb
PDB entry 5l4k coloured by chain, front view.
PDB entry 5l4k coloured by chain, side view.
PDB entry 5l4k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human 26S proteasome at a resolution of 3.9 Å.
Schweitzer A, Aufderheide A, Rudack T, Beck F, Pfeifer G, Plitzko JM, Sakata E, Schulten K, Förster F, Baumeister W
Proc Natl Acad Sci U S A 113 7816-21 (2016)
PMID: 27342858
Related structures: EMD-4002

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126190 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
26S proteasome non-ATPase regulatory subunit 4 Chain: W
Molecule details ›
Chain: W
Length: 377 amino acids
Theoretical weight: 40.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P55036 (Residues: 1-377; Coverage: 100%)
Gene names: MCB1, PSMD4
Sequence domains:
26S proteasome non-ATPase regulatory subunit 14 Chain: V
Molecule details ›
Chain: V
Length: 310 amino acids
Theoretical weight: 34.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00487 (Residues: 1-310; Coverage: 100%)
Gene names: POH1, PSMD14
Sequence domains: JAB1/Mov34/MPN/PAD-1 ubiquitin protease
26S proteasome non-ATPase regulatory subunit 8 Chain: T
Molecule details ›
Chain: T
Length: 350 amino acids
Theoretical weight: 39.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P48556 (Residues: 1-350; Coverage: 100%)
Gene name: PSMD8
Sequence domains: CSN8/PSMD8/EIF3K family
26S proteasome complex subunit SEM1 Chain: Y
Molecule details ›
Chain: Y
Length: 70 amino acids
Theoretical weight: 8.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60896 (Residues: 1-70; Coverage: 100%)
Gene names: C7orf76, DSS1, SEM1, SHFDG1, SHFM1
Sequence domains: DSS1/SEM1 family
26S proteasome non-ATPase regulatory subunit 2 Chain: Z
Molecule details ›
Chain: Z
Length: 908 amino acids
Theoretical weight: 100.31 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13200 (Residues: 1-908; Coverage: 100%)
Gene names: PSMD2, TRAP2
Sequence domains:
26S proteasome non-ATPase regulatory subunit 1 Chain: N
Molecule details ›
Chain: N
Length: 953 amino acids
Theoretical weight: 105.96 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99460 (Residues: 1-953; Coverage: 100%)
Gene name: PSMD1
Sequence domains:
26S proteasome non-ATPase regulatory subunit 3 Chain: S
Molecule details ›
Chain: S
Length: 534 amino acids
Theoretical weight: 61.07 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O43242 (Residues: 1-534; Coverage: 100%)
Gene name: PSMD3
Sequence domains:
26S proteasome non-ATPase regulatory subunit 12 Chain: P
Molecule details ›
Chain: P
Length: 456 amino acids
Theoretical weight: 52.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00232 (Residues: 1-456; Coverage: 100%)
Gene name: PSMD12
Sequence domains:
26S proteasome non-ATPase regulatory subunit 11 Chain: Q
Molecule details ›
Chain: Q
Length: 422 amino acids
Theoretical weight: 47.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00231 (Residues: 1-422; Coverage: 100%)
Gene name: PSMD11
Sequence domains:
26S proteasome non-ATPase regulatory subunit 6 Chain: R
Molecule details ›
Chain: R
Length: 389 amino acids
Theoretical weight: 45.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15008 (Residues: 1-389; Coverage: 100%)
Gene names: KIAA0107, PFAAP4, PSMD6
Sequence domains:
26S proteasome non-ATPase regulatory subunit 7 Chain: U
Molecule details ›
Chain: U
Length: 324 amino acids
Theoretical weight: 37.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P51665 (Residues: 1-324; Coverage: 100%)
Gene names: MOV34L, PSMD7
Sequence domains:
26S proteasome non-ATPase regulatory subunit 13 Chain: O
Molecule details ›
Chain: O
Length: 376 amino acids
Theoretical weight: 43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UNM6 (Residues: 1-376; Coverage: 100%)
Gene name: PSMD13
Sequence domains: PCI domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-4002

Quick links

Citations

37 review citations

The Logic of the 26S Proteasome.
Collins et al. (2017)
36 more

6 mentions without citation

Structure of the human 26S proteasome at a resolution of 3.9 Å.
Schweitzer et al. (2016)
5 more