› 5l6w

X-ray diffraction
2.53Å resolution

Structure Of the LIMK1-ATPgammaS-CFL1 Complex

Released:
DOI: 10.2210/pdb5l6w/pdb
PDB entry 5l6w coloured by chain, front view.
PDB entry 5l6w coloured by chain, side view.
PDB entry 5l6w coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Salah E, Mathea S, Oerum S, Newman JA, Tallant C, Adamson R, Canning P, Beltrami A, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Knapp S, Bullock AN

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149995 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
LIM domain kinase 1 Chain: L
Molecule details ›
Chain: L
Length: 310 amino acids
Theoretical weight: 35.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53667 (Residues: 330-637; Coverage: 48%)
Gene names: LIMK, LIMK1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Cofilin-1 Chain: C
Molecule details ›
Chain: C
Length: 167 amino acids
Theoretical weight: 18.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23528 (Residues: 1-166; Coverage: 100%)
Gene names: CFL, CFL1
Sequence domains: Cofilin/tropomyosin-type actin-binding protein
Structure domains: Severin

Ligands and Environments

1 bound ligand:
Ligand AGS 1 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3221
Unit cell:
a: 80.679Å b: 80.679Å c: 237.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.226 0.284
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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