› 5l7d

X-ray diffraction
3.2Å resolution

Structure of human Smoothened in complex with cholesterol

Released:
DOI: 10.2210/pdb5l7d/pdb
PDB entry 5l7d coloured by chain, front view.
PDB entry 5l7d coloured by chain, side view.
PDB entry 5l7d coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of Smoothened regulation by its extracellular domains.
Byrne EFX, Sircar R, Miller PS, Hedger G, Luchetti G, Nachtergaele S, Tully MD, Mydock-McGrane L, Covey DF, Rambo RP, Sansom MSP, Newstead S, Rohatgi R, Siebold C
Nature 535 517-522 (2016)
PMID: 27437577

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein smoothened; Soluble cytochrome b562 Chains: A, B
Molecule details ›
Chains: A, B
Length: 638 amino acids
Theoretical weight: 70.78 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: Q99835 (Residues: 32-428, 443-555; Coverage: 67%)
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
Gene names: SMO, SMOH, cybC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand CLR 1 x CLR
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 122.9Å b: 63.02Å c: 208.59Å
α: 90° β: 96.64° γ: 90°
R-values:
R R work R free
0.232 0.23 0.264
Expression system: Homo sapiens

Quick links

Citations

51 review citations

Hedgehog Signaling: From Basic Biology to Cancer Therapy.
Wu et al. (2017)
50 more

48 mentions without citation

Wnt signaling in triple-negative breast cancer.
Pohl et al. (2017)
47 more