› 5l8e

X-ray diffraction
2.3Å resolution

Structure of UAF1

Released:
DOI: 10.2210/pdb5l8e/pdb
PDB entry 5l8e coloured by chain, front view.
PDB entry 5l8e coloured by chain, side view.
PDB entry 5l8e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme.
Dharadhar S, Clerici M, van Dijk WJ, Fish A, Sixma TK
J Struct Biol 196 437-447 (2016)
PMID: 27650958

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-165934 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 48 Chains: A, B
Molecule details ›
Chains: A, B
Length: 580 amino acids
Theoretical weight: 65.19 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TAF3 (Residues: 9-580; Coverage: 85%)
Gene names: KIAA1449, UAF1, WDR48
Sequence domains:
Unknown Chain: C
Molecule details ›
Chain: C
Length: 5 amino acids
Theoretical weight: 444 Da
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand GOL 14 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Unit cell:
a: 73.303Å b: 131.601Å c: 148.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.224
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Mechanisms of Deubiquitinase Specificity and Regulation.
Mevissen et al. (2017)
3 more

2 mentions without citation

A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme.
Dharadhar et al. (2016)
1 more