› 5le0

X-ray diffraction
3.3Å resolution

MICAL1 Cterminal domain

Released:
DOI: 10.2210/pdb5le0/pdb
PDB entry 5le0 coloured by chain, front view.
PDB entry 5le0 coloured by chain, side view.
PDB entry 5le0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Oxidation of F-actin controls the terminal steps of cytokinesis.
Frémont S, Hammich H, Bai J, Wioland H, Klinkert K, Rocancourt M, Kikuti C, Stroebel D, Romet-Lemonne G, Pylypenko O, Houdusse A, Echard A
Nat Commun 8 14528 (2017)
PMID: 28230050

Function and Biology Details

Reactions catalysed: 
[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine-(R)-S-oxide + NADP(+) + H(2)O
NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[F-actin]-monooxygenase MICAL1 Chain: B
Molecule details ›
Chain: B
Length: 150 amino acids
Theoretical weight: 17.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TDZ2 (Residues: 918-1067; Coverage: 14%)
Gene names: MICAL, MICAL1, NICAL
Sequence domains: Bivalent Mical/EHBP Rab binding domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P4322
Unit cell:
a: 52.78Å b: 52.78Å c: 157Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.271 0.306
Expression system: Escherichia coli

Quick links

Citations

28 review citations

The many functions of ESCRTs.
Vietri et al. (2020)
27 more

1 mention without citation

Oxidation of F-actin controls the terminal steps of cytokinesis.
Frémont et al. (2017)