› 5lgg

X-ray diffraction
2.15Å resolution

The N-terminal WD40 domain of Apc1 (Anaphase promoting complex subunit 1)

Released:
DOI: 10.2210/pdb5lgg/pdb
PDB entry 5lgg coloured by chain, front view.
PDB entry 5lgg coloured by chain, side view.
PDB entry 5lgg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
WD40 domain of Apc1 is critical for the coactivator-induced allosteric transition that stimulates APC/C catalytic activity.
Li Q, Chang L, Aibara S, Yang J, Zhang Z, Barford D
Proc Natl Acad Sci U S A 113 10547-52 (2016)
PMID: 27601667

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anaphase-promoting complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 47.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9H1A4 (Residues: 1-33, 70-306, 403-613; Coverage: 22%)
Gene names: ANAPC1, TSG24
Sequence domains: Anaphase-promoting complex subunit 1 WD40 beta-propeller domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2221
Unit cell:
a: 82.633Å b: 113.262Å c: 100.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.226
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C).
Alfieri et al. (2017)
3 more

1 mention without citation

WD40 domain of Apc1 is critical for the coactivator-induced allosteric transition that stimulates APC/C catalytic activity.
Li et al. (2016)