› 5lhy

X-ray diffraction
3.31Å resolution

PB3 Domain of Human PLK4 (apo)

Released:
DOI: 10.2210/pdb5lhy/pdb
PDB entry 5lhy coloured by chain, front view.
PDB entry 5lhy coloured by chain, side view.
PDB entry 5lhy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A key centriole assembly interaction interface between human PLK4 and STIL appears to not be conserved in flies.
Cottee MA, Johnson S, Raff JW, Lea SM
Biol Open 6 381-389 (2017)
PMID: 28202467

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK4 Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x, y
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x, y
Length: 91 amino acids
Theoretical weight: 9.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli B
UniProt:
  • Canonical: O00444 (Residues: 884-970; Coverage: 9%)
Gene names: PLK4, SAK, STK18
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 220.35Å b: 220.35Å c: 325.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.269 0.295
Expression system: Escherichia coli B

Quick links

Citations

9 citation in other articles

Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation.
Dzhindzhev et al. (2017)
8 more