› 5lp0

X-ray diffraction
1.41Å resolution

CRYSTAL STRUCTURE OF THE ZEBRA FISH ENTH DOMAIN FROM EPSIN1 IN 1.41 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb5lp0/pdb
PDB entry 5lp0 coloured by chain, front view.
PDB entry 5lp0 coloured by chain, side view.
PDB entry 5lp0 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
A bacterial genetic selection system for ubiquitylation cascade discovery.
Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T, Katina C, Pilzer I, Ben-Aroya S, Prag G
Nat Methods 13 945-952 (2016)
PMID: 27694912

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123335 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ENTH domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 145 amino acids
Theoretical weight: 16.77 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F1Q5Z9 (Residues: 18-157; Coverage: 22%)
Sequence domains: ENTH domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 42.364Å b: 51.724Å c: 64.639Å
α: 89.33° β: 76.37° γ: 79.74°
R-values:
R R work R free
0.157 0.154 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Protein Engineering in the Ubiquitin System: Tools for Discovery and Beyond.
Zhao et al. (2020)
1 more

3 mentions without citation

Structure and evolution of ENTH and VHS/ENTH-like domains in tepsin.
Archuleta et al. (2017)
2 more