› 5ls6

X-ray diffraction
3.47Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131030 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone-lysine N-methyltransferase EZH2 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 695 amino acids
Theoretical weight: 79.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q15910 (Residues: 1-385, 421-746; Coverage: 92%)
Gene names: EZH2, KMT6
Sequence domains:
Polycomb protein EED Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 367 amino acids
Theoretical weight: 42.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O75530 (Residues: 77-441; Coverage: 83%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Polycomb protein SUZ12 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 129 amino acids
Theoretical weight: 15.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q15022 (Residues: 558-685; Coverage: 17%)
Gene names: CHET9, JJAZ1, KIAA0160, SUZ12
Sequence domains: VEFS-Box of polycomb protein
Protein Jumonji Chains: Q, R, S, T
Molecule details ›
Chains: Q, R, S, T
Length: 11 amino acids
Theoretical weight: 1.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q92833 (Residues: 110-120; Coverage: 1%)
Gene names: JARID2, JMJ

Ligands and Environments

2 bound ligands:
Ligand ZN 32 x ZN
Ligand 74D 4 x 74D
1 modified residue:
Ligand M3L 4 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 131.31Å b: 170.27Å c: 275.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.299
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

50 review citations

The great escape: tumour cell plasticity in resistance to targeted therapy.
Boumahdi et al. (2020)
49 more

16 mentions without citation

Molecular Mechanisms Directing PRC2 Recruitment and H3K27 Methylation.
Laugesen et al. (2019)
15 more