› 5lsi

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE KINETOCHORE MIS12 COMPLEX HEAD2 SUBDOMAIN CONTAINING DSN1 AND NSL1 FRAGMENTS

Released:
DOI: 10.2210/pdb5lsi/pdb
PDB entry 5lsi coloured by chain, front view.
PDB entry 5lsi coloured by chain, side view.
PDB entry 5lsi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A
Cell 167 1028-1040.e15 (2016)
PMID: 27881301

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188549 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kinetochore-associated protein DSN1 homolog Chain: D
Molecule details ›
Chain: D
Length: 134 amino acids
Theoretical weight: 15.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H410 (Residues: 67-200; Coverage: 38%)
Gene names: C20orf172, DSN1, MIS13
Sequence domains: Mis12-Mtw1 protein family
Kinetochore-associated protein NSL1 homolog Chain: E
Molecule details ›
Chain: E
Length: 76 amino acids
Theoretical weight: 8.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96IY1 (Residues: 29-99; Coverage: 25%)
Gene names: C1orf48, DC31, DC8, MIS14, NSL1

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P3221
Unit cell:
a: 59.64Å b: 59.64Å c: 82.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.216 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

A Molecular View of Kinetochore Assembly and Function.
Musacchio et al. (2017)
20 more

2 mentions without citation

Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Petrovic et al. (2016)
1 more