› 5lsk

X-ray diffraction
3.5Å resolution

CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE MIS12-CENP-C COMPLEX

Released:
DOI: 10.2210/pdb5lsk/pdb
PDB entry 5lsk coloured by chain, front view.
PDB entry 5lsk coloured by chain, side view.
PDB entry 5lsk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A
Cell 167 1028-1040.e15 (2016)
PMID: 27881301

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-169866 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Protein MIS12 homolog Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H081 (Residues: 1-205; Coverage: 100%)
Gene name: MIS12
Sequence domains: Mis12 protein
Polyamine-modulated factor 1 Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P1K2 (Residues: 31-205; Coverage: 85%)
Gene name: PMF1
Sequence domains: Nnf1
Kinetochore-associated protein DSN1 homolog Chain: D
Molecule details ›
Chain: D
Length: 296 amino acids
Theoretical weight: 33.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H410 (Residues: 67-356; Coverage: 82%)
Gene names: C20orf172, DSN1, MIS13
Sequence domains: Mis12-Mtw1 protein family
Kinetochore-associated protein NSL1 homolog Chain: N
Molecule details ›
Chain: N
Length: 206 amino acids
Theoretical weight: 23.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96IY1 (Residues: 1-206; Coverage: 73%)
Gene names: C1orf48, DC31, DC8, MIS14, NSL1
Sequence domains: Kinetochore protein Mis14 like
Centromere protein C Chain: P
Molecule details ›
Chain: P
Length: 76 amino acids
Theoretical weight: 8.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03188 (Residues: 1-71; Coverage: 8%)
Gene names: CENPC, CENPC1, ICEN7

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 146.01Å b: 112.69Å c: 90.81Å
α: 90° β: 114.18° γ: 90°
R-values:
R R work R free
0.247 0.244 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

A Molecular View of Kinetochore Assembly and Function.
Musacchio et al. (2017)
16 more

4 mentions without citation

Seeing is believing: our evolving view of kinetochore structure, composition, and assembly.
Hamilton et al. (2019)
3 more