› 5ltw

X-ray diffraction
4.5Å resolution

Complex of human 14-3-3 sigma CLU1 mutant with phosphorylated heat shock protein B6

Released:
DOI: 10.2210/pdb5ltw/pdb
PDB entry 5ltw coloured by chain, front view.
PDB entry 5ltw coloured by chain, side view.
PDB entry 5ltw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for the Interaction of a Human Small Heat Shock Protein with the 14-3-3 Universal Signaling Regulator.
Sluchanko NN, Beelen S, Kulikova AA, Weeks SD, Antson AA, Gusev NB, Strelkov SV
Structure 25 305-316 (2017)
PMID: 28089448

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127006 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chains: A, B, E, F, I, J
Molecule details ›
Chains: A, B, E, F, I, J
Length: 234 amino acids
Theoretical weight: 26.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Heat shock protein beta-6 Chains: C, D, G, H, K, L
Molecule details ›
Chains: C, D, G, H, K, L
Length: 149 amino acids
Theoretical weight: 16.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14558 (Residues: 1-149; Coverage: 93%)
Gene name: HSPB6
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand TRS 6 x TRS
Ligand NI 3 x NI
1 modified residue:
Ligand SEP 6 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2221
Unit cell:
a: 125.06Å b: 341.29Å c: 144.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.236 0.256
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The dynamic and stress-adaptive signaling hub of 14-3-3: emerging mechanisms of regulation and context-dependent protein-protein interactions.
Pennington et al. (2018)
10 more

14 mentions without citation

Modulators of 14-3-3 Protein-Protein Interactions.
Stevers et al. (2018)
13 more