› 5lv0

X-ray diffraction
2.7Å resolution

Structure of Human Neurolysin (E475Q) in complex with amyloid-beta 35-40 peptide product

Released:
DOI: 10.2210/pdb5lv0/pdb
PDB entry 5lv0 coloured by chain, front view.
PDB entry 5lv0 coloured by chain, side view.
PDB entry 5lv0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Masuyer G, Berntsson RP-A, Teixeira PF, Kmiec B, Glaser E, Stenmark P

Function and Biology Details

Reaction catalysed: 
Preferential cleavage in neurotensin: 10-Pro-|-Tyr-11.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138371 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neurolysin, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 686 amino acids
Theoretical weight: 78.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BYT8 (Residues: 38-704; Coverage: 95%)
Gene names: AGTBP, KIAA1226, NLN
Sequence domains: Peptidase family M3
Structure domains:
P3(40) Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 561 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 706-711; Coverage: 1%)
Gene names: A4, AD1, APP

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P43212
Unit cell:
a: 131.341Å b: 131.341Å c: 195.979Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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