› 5lyg

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF INTRACELLULAR B30.2 DOMAIN OF BTN3A1 BOUND TO MALONATE

Released:
DOI: 10.2210/pdb5lyg/pdb
PDB entry 5lyg coloured by chain, front view.
PDB entry 5lyg coloured by chain, side view.
PDB entry 5lyg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
BTN3A1 Discriminates γδ T Cell Phosphoantigens from Nonantigenic Small Molecules via a Conformational Sensor in Its B30.2 Domain.
Salim M, Knowles TJ, Baker AT, Davey MS, Jeeves M, Sridhar P, Wilkie J, Willcox CR, Kadri H, Taher TE, Vantourout P, Hayday A, Mehellou Y, Mohammed F, Willcox BE
ACS Chem Biol 12 2631-2643 (2017)
PMID: 28862425

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Butyrophilin subfamily 3 member A1 Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 21.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00481 (Residues: 327-513; Coverage: 39%)
Gene names: BTF5, BTN3A1
Sequence domains:
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand MLI 1 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21212
Unit cell:
a: 45.73Å b: 127.22Å c: 39.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Thymic development of unconventional T cells: how NKT cells, MAIT cells and γδ T cells emerge.
Pellicci et al. (2020)
13 more

2 mentions without citation

BTN3A1 Discriminates γδ T Cell Phosphoantigens from Nonantigenic Small Molecules via a Conformational Sensor in Its B30.2 Domain.
Salim et al. (2017)
1 more