› 5lz5

X-ray diffraction
2.05Å resolution

Function and Biology Details

Reaction catalysed: 
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Platelet-activating factor acetylhydrolase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 44.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13093 (Residues: 46-428; Coverage: 91%)
Gene names: PAFAH, PLA2G7
Sequence domains: Platelet-activating factor acetylhydrolase, isoform II
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand 7K4 1 x 7K4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 101.091Å b: 91.494Å c: 48.648Å
α: 90° β: 111.92° γ: 90°
R-values:
R R work R free
0.186 0.184 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Small-molecule inhibitors as potential therapeutics and as tools to understand the role of phospholipases A2.
Nikolaou et al. (2019)
2 more