› 5m1x

X-ray diffraction
1.8Å resolution

Crystal structure of S. cerevisiae Rfa1 N-OB domain mutant (K45E)

Released:
DOI: 10.2210/pdb5m1x/pdb
PDB entry 5m1x coloured by chain, front view.
PDB entry 5m1x coloured by chain, side view.
PDB entry 5m1x coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to Hold Sister Chromatids Together.
Seeber A, Hegnauer AM, Hustedt N, Deshpande I, Poli J, Eglinger J, Pasero P, Gut H, Shinohara M, Hopfner KP, Shimada K, Gasser SM
Mol. Cell 64 951-966 (2016)
PMID: 27889450

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication factor A protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 134 amino acids
Theoretical weight: 15.62 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22336 (Residues: 1-132; Coverage: 21%)
Gene names: BUF2, FUN3, RFA1, RPA1, YAR007C

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 29.62Å b: 115.35Å c: 69.03Å
α: 90° β: 90.71° γ: 90°
R-values:
R R work R free
0.167 0.164 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Replication-Coupled DNA Repair.
Cortez D. (2019)
8 more