› 5m25

X-ray diffraction
2.43Å resolution

Modulation of MLL1 Methyltransferase Activity

Released:
DOI: 10.2210/pdb5m25/pdb
PDB entry 5m25 coloured by chain, front view.
PDB entry 5m25 coloured by chain, side view.
PDB entry 5m25 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Controlled inhibition of methyltransferases using photoswitchable peptidomimetics: towards an epigenetic regulation of leukemia.
Albert L, Xu J, Wan R, Srinivasan V, Dou Y, Vázquez O
Chem Sci 8 4612-4618 (2017)
PMID: 28970883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 48.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P61964 (Residues: 22-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand 7DU 1 x 7DU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 80.714Å b: 85.917Å c: 39.276Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.176 0.248
Expression system: Escherichia coli K-12

Quick links

Citations

5 review citations

Recent Implementations of Molecular Photoswitches into Smart Materials and Biological Systems.
Pianowski ZL. (2019)
4 more

1 mention without citation

Controlled inhibition of methyltransferases using photoswitchable peptidomimetics: towards an epigenetic regulation of leukemia.
Albert et al. (2017)