› 5m2m

X-ray diffraction
2.3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208424 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor, soluble form Chains: A, B, C, G, I, M
Molecule details ›
Chains: A, B, C, G, I, M
Length: 157 amino acids
Theoretical weight: 17.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01375 (Residues: 77-233; Coverage: 67%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Llama nanobody VHH3 Chains: D, E, F, H, J, N
Molecule details ›
Chains: D, E, F, H, J, N
Length: 129 amino acids
Theoretical weight: 14.2 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2
Unit cell:
a: 145.38Å b: 83.844Å c: 150.063Å
α: 90° β: 128.77° γ: 90°
R-values:
R R work R free
0.213 0.211 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

A New Venue of TNF Targeting.
Steeland et al. (2018)
7 more

2 mentions without citation

Bivalent Llama Single-Domain Antibody Fragments against Tumor Necrosis Factor Have Picomolar Potencies due to Intramolecular Interactions.
Beirnaert et al. (2017)
1 more