› 5m56

X-ray diffraction
2.24Å resolution

Monoclinic complex structure of human protein kinase CK2 catalytic subunit (isoform CK2alpha') with the inhibitor 4'-carboxy-6,8-chloro-flavonol (FLC21)

Released:
DOI: 10.2210/pdb5m56/pdb
PDB entry 5m56 coloured by chain, front view.
PDB entry 5m56 coloured by chain, side view.
PDB entry 5m56 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Hypervariability of the Two Human Protein Kinase CK2 Catalytic Subunit Paralogs Revealed by Complex Structures with a Flavonol- and a Thieno[2,3-d]pyrimidine-Based Inhibitor.
Niefind K, Bischoff N, Golub AG, Bdzhola VG, Balanda AO, Prykhod'ko AO, Yarmoluk SM
Pharmaceuticals (Basel) 10 (2017)
PMID: 28085026

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148708 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha' Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 42.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19784 (Residues: 1-350; Coverage: 100%)
Gene names: CK2A2, CSNK2A2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 7FC 2 x 7FC
Ligand GOL 4 x GOL
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 69.341Å b: 87.62Å c: 72.977Å
α: 90° β: 109.69° γ: 90°
R-values:
R R work R free
0.165 0.164 0.206
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Conformational dynamics of human protein kinase CK2α and its effect on function and inhibition.
Srivastava et al. (2018)
4 more

3 mentions without citation

Structural Hypervariability of the Two Human Protein Kinase CK2 Catalytic Subunit Paralogs Revealed by Complex Structures with a Flavonol- and a Thieno[2,3-d]pyrimidine-Based Inhibitor.
Niefind et al. (2017)
2 more