› 5mam

X-ray diffraction
2.2Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: 0, 2, 4, A, C, E, G, I, K, M, O, Q, S, U, W, Y
Molecule details ›
Chains: 0, 2, 4, A, C, E, G, I, K, M, O, Q, S, U, W, Y
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: 1, 3, 5, B, D, F, H, J, L, N, P, R, T, V, X, Z
Molecule details ›
Chains: 1, 3, 5, B, D, F, H, J, L, N, P, R, T, V, X, Z
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand CL 8 x CL
Ligand SRO 14 x SRO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R3
Unit cell:
a: 158.96Å b: 158.96Å c: 76.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.202 0.28
Expression system: Not provided

Quick links

Citations

2 review citations

Progress in Simulation Studies of Insulin Structure and Function.
Gorai et al. (2022)
1 more

1 mention without citation

Computational and structural evidence for neurotransmitter-mediated modulation of the oligomeric states of human insulin in storage granules.
Palivec et al. (2017)