› 5mav

X-ray diffraction
2.58Å resolution

Crystal structure of human PCNA in complex with PARG (poly(ADP-ribose) glycohydrolase) peptide.

Released:
DOI: 10.2210/pdb5mav/pdb
PDB entry 5mav coloured by chain, front view.
PDB entry 5mav coloured by chain, side view.
PDB entry 5mav coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel non-canonical PIP-box mediates PARG interaction with PCNA.
Kaufmann T, Grishkovskaya I, Polyansky AA, Kostrhon S, Kukolj E, Olek KM, Herbert S, Beltzung E, Mechtler K, Peterbauer T, Gotzmann J, Zhang L, Hartl M, Zagrovic B, Elsayad K, Djinovic-Carugo K, Slade D
Nucleic Acids Res 45 9741-9759 (2017)
PMID: 28934471

Function and Biology Details

Reaction catalysed: 
Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146057 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 264 amino acids
Theoretical weight: 29.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
poly(ADP-ribose) glycohydrolase Chains: G, H, K, L, M, N
Molecule details ›
Chains: G, H, K, L, M, N
Length: 19 amino acids
Theoretical weight: 2.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q0MQR4 (Residues: 402-420; Coverage: 2%)
Gene name: PARG

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 77.597Å b: 153.604Å c: 85.512Å
α: 90° β: 96.9° γ: 90°
R-values:
R R work R free
0.241 0.239 0.291
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

PARP and PARG inhibitors in cancer treatment.
Slade D. (2020)
8 more

5 mentions without citation

Human PCNA Structure, Function and Interactions.
González-Magaña et al. (2020)
4 more