› 5mf7

X-ray diffraction
1.59Å resolution

New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-GADD45)

Released:
DOI: 10.2210/pdb5mf7/pdb
PDB entry 5mf7 coloured by chain, front view.
PDB entry 5mf7 coloured by chain, side view.
PDB entry 5mf7 coloured by chain, top view.
Source organisms:
Primary publication:
New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins.
Golovenko D, Bräuning B, Vyas P, Haran TE, Rozenberg H, Shakked Z
Structure 26 1237-1250.e6 (2018)
PMID: 30057026

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119022 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 138.056Å b: 50.034Å c: 67.202Å
α: 90° β: 92.52° γ: 90°
R-values:
R R work R free
0.201 0.198 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Targeting p53 pathways: mechanisms, structures, and advances in therapy.
Wang et al. (2023)
21 other articles

3 mentions without citation

Facilitated Diffusion Mechanisms in DNA Base Excision Repair and Transcriptional Activation.
Esadze et al. (2018)
2 more