› 5mg7

X-ray diffraction
1.45Å resolution

New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-p53R2)

Released:
DOI: 10.2210/pdb5mg7/pdb
PDB entry 5mg7 coloured by chain, front view.
PDB entry 5mg7 coloured by chain, side view.
PDB entry 5mg7 coloured by chain, top view.
Source organisms:
Primary publication:
New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins.
Golovenko D, Bräuning B, Vyas P, Haran TE, Rozenberg H, Shakked Z
Structure 26 1237-1250.e6 (2018)
PMID: 30057026

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119023 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 137.621Å b: 49.804Å c: 68.089Å
α: 90° β: 93.34° γ: 90°
R-values:
R R work R free
0.172 0.171 0.202
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Facilitated Diffusion Mechanisms in DNA Base Excision Repair and Transcriptional Activation.
Esadze et al. (2018)
1 more

2 mentions without citation

Deep Molecular and In Silico Protein Analysis of p53 Alteration in Myelodysplastic Neoplasia and Acute Myeloid Leukemia.
Madarász et al. (2022)
1 more