› 5mhl

X-ray diffraction
2.4Å resolution

FXIIIa in complex with the inhibitor Mi0621

Released:
DOI: 10.2210/pdb5mhl/pdb
PDB entry 5mhl coloured by chain, front view.
PDB entry 5mhl coloured by chain, side view.
PDB entry 5mhl coloured by chain, top view.
Source organisms:
Entry authors: Stieler M, Heine A, Klebe G

Function and Biology Details

Reaction catalysed: 
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132550 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Coagulation factor XIII A chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 738 amino acids
Theoretical weight: 84.21 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00488 (Residues: 2-732; Coverage: 100%)
Gene names: F13A, F13A1
Sequence domains:
Structure domains:
inhibitor Mi0621 Chains: K, O
Molecule details ›
Chains: K, O
Length: 9 amino acids
Theoretical weight: 928 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand CA 6 x CA
Ligand GOL 5 x GOL
Ligand EDO 1 x EDO
Ligand SO4 4 x SO4
1 modified residue:
Ligand ONL 2 x ONL

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 57.213Å b: 80.993Å c: 103.329Å
α: 88.12° β: 76.87° γ: 81.47°
R-values:
R R work R free
0.175 0.172 0.23
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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