› 5mio

X-ray diffraction
3.19Å resolution

KIF2C-DARPIN FUSION PROTEIN BOUND TO TUBULIN

Released:
DOI: 10.2210/pdb5mio/pdb
PDB entry 5mio coloured by chain, front view.
PDB entry 5mio coloured by chain, side view.
PDB entry 5mio coloured by chain, top view.
Source organisms:
Primary publication:
Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin.
Wang W, Cantos-Fernandes S, Lv Y, Kuerban H, Ahmad S, Wang C, Gigant B
Nat Commun 8 70 (2017)
PMID: 28694425

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189238 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Kinesin-like protein KIF2C Chain: C
Molecule details ›
Chain: C
Length: 573 amino acids
Theoretical weight: 62.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99661 (Residues: 216-598; Coverage: 53%)
Gene names: KIF2C, KNSL6
Sequence domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 2 x MG
Ligand GDP 1 x GDP
Ligand LOC 1 x LOC
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 51.81Å b: 229.76Å c: 293.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.257
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Microtubule dynamics: an interplay of biochemistry and mechanics.
Brouhard et al. (2018)
5 more

11 mentions without citation

Parts list for a microtubule depolymerising kinesin.
Friel et al. (2018)
10 more