› 5mk1

X-ray diffraction
2.5Å resolution

Crystal structure of the His Domain Protein Tyrosine Phosphatase (HD-PTP/PTPN23) Bro1 domain (CHMP4A peptide complex structure)

Released:
DOI: 10.2210/pdb5mk1/pdb
PDB entry 5mk1 coloured by chain, front view.
PDB entry 5mk1 coloured by chain, side view.
PDB entry 5mk1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.
Gahloth D, Levy C, Walker L, Wunderley L, Mould AP, Taylor S, Woodman P, Tabernero L
Structure 25 1011-1024.e4 (2017)
PMID: 28602823

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189817 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 23 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 361 amino acids
Theoretical weight: 40.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H3S7 (Residues: 1-361; Coverage: 22%)
Gene names: KIAA1471, PTPN23
Sequence domains: BRO1-like domain
Structure domains: alix/aip1 like domains
Charged multivesicular body protein 4a Chains: E, F, H, K
Molecule details ›
Chains: E, F, H, K
Length: 18 amino acids
Theoretical weight: 2.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9BY43 (Residues: 205-222; Coverage: 8%)
Gene names: C14orf123, CDA04, CHMP4A, HSPC134, SHAX2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 71.458Å b: 73.669Å c: 79.383Å
α: 114.43° β: 90.28° γ: 104.2°
R-values:
R R work R free
0.178 0.176 0.226
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Dissecting the role of His domain protein tyrosine phosphatase/PTPN23 and ESCRTs in sorting activated epidermal growth factor receptor to the multivesicular body.
Tabernero et al. (2018)
1 more

1 mention without citation

Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.
Gahloth et al. (2017)