› 5mk5

X-ray diffraction
2.16Å resolution

Structures of DHBN domain of human BLM helicase

Released:
DOI: 10.2210/pdb5mk5/pdb
PDB entry 5mk5 coloured by chain, front view.
PDB entry 5mk5 coloured by chain, side view.
PDB entry 5mk5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A helical bundle in the N-terminal domain of the BLM helicase mediates dimer and potentially hexamer formation.
Shi J, Chen WF, Zhang B, Fan SH, Ai X, Liu NN, Rety S, Xi XG
J Biol Chem 292 5909-5920 (2017)
PMID: 28228481

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RecQ-like DNA helicase BLM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 54 amino acids
Theoretical weight: 6.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54132 (Residues: 362-414; Coverage: 4%)
Gene names: BLM, RECQ2, RECQL3
Sequence domains: BDHCT (NUC031) domain

Ligands and Environments

2 bound ligands:
Ligand IOD 20 x IOD
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2221
Unit cell:
a: 34.082Å b: 144.729Å c: 96.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Human RecQ Helicases in DNA Double-Strand Break Repair.
Lu et al. (2021)
1 more

2 mentions without citation

COVID-19 Outbreak: An Overview.
Ciotti et al. (2019)
1 more