› 5mlb

X-ray diffraction
3.22Å resolution

Crystal structure of human RAS in complex with darpin K27

Released:
DOI: 10.2210/pdb5mlb/pdb
PDB entry 5mlb coloured by chain, front view.
PDB entry 5mlb coloured by chain, side view.
PDB entry 5mlb coloured by chain, top view.
Source organisms:
Entry authors: Debreczeni JE, Guillard S, Kolasinska-Zwierz P, Breed J, Zhang J, Bery N, Marwood R, Tart J, Overman R, Stocki P, Mistry B, Phillips C, Rabbitts T, Jackson R, Minter R

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134002 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase KRas, N-terminally processed Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 169 amino acids
Theoretical weight: 19.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-166; Coverage: 88%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
DARPin K27 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 178 amino acids
Theoretical weight: 19.53 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
Ligand GDP 4 x GDP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P65
Unit cell:
a: 197.14Å b: 197.14Å c: 84.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.235
Expression system: Escherichia coli

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