› 5mp0

X-ray diffraction
1.63Å resolution

Human m7GpppN-mRNA Hydrolase (DCP2, NUDT20) Catalytic Domain

Released:
DOI: 10.2210/pdb5mp0/pdb
PDB entry 5mp0 coloured by chain, front view.
PDB entry 5mp0 coloured by chain, side view.
PDB entry 5mp0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Mathea S, Salah E, Velupillai S, Tallant C, Pike ACW, Bushell SR, Faust B, Wang D, Burgess-Brown N, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Knapp S, Huber K

Function and Biology Details

Reaction catalysed: 
5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] + H(2)O = N(7)-methylguanosine 5'-diphosphate + 5'-phospho-[mRNA]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184843 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
m7GpppN-mRNA hydrolase Chain: D
Molecule details ›
Chain: D
Length: 166 amino acids
Theoretical weight: 18.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8IU60 (Residues: 95-260; Coverage: 40%)
Gene names: DCP2, NUDT20
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 48.05Å b: 60.57Å c: 65.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.262
Expression system: Escherichia coli BL21

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