› 5mpo

X-ray diffraction
2.43Å resolution

Crystal structure of human molybdopterin synthase complex

Released:
DOI: 10.2210/pdb5mpo/pdb
PDB entry 5mpo coloured by chain, front view.
PDB entry 5mpo coloured by chain, side view.
PDB entry 5mpo coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kopec J, Bailey H, Fitzpatrick F, Strain-Damerell C, Oberholzer AE, Williams E, Burgess-Brown N, von Delft F, Arrowsmith C, Edwards A, Bountra C, Yue WW

Function and Biology Details

Reaction catalysed: 
Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + H(2)O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132111 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Molybdopterin synthase sulfur carrier subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.84 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O96033 (Residues: 7-88; Coverage: 93%)
Gene names: MOCO1, MOCS2
Sequence domains: ThiS family
Structure domains: Ubiquitin-like (UB roll)
Molybdopterin synthase catalytic subunit Chains: C, D
Molecule details ›
Chains: C, D
Length: 154 amino acids
Theoretical weight: 17.25 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O96007 (Residues: 27-179; Coverage: 81%)
Gene names: MCBPE, MOCO1, MOCS2
Sequence domains: MoaE protein
Structure domains: Molybdopterin biosynthesis MoaE subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P65
Unit cell:
a: 123.72Å b: 123.72Å c: 82.49Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.259
Expression system: Spodoptera frugiperda

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