› 5mpr

X-ray diffraction
1.6Å resolution

Single Amino Acid Variant of Human Mitochondrial Branched Chain Amino Acid Aminotransferase 2

Released:
DOI: 10.2210/pdb5mpr/pdb
PDB entry 5mpr coloured by chain, front view.
PDB entry 5mpr coloured by chain, side view.
PDB entry 5mpr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Intact Protein Analysis at 21 Tesla and X-Ray Crystallography Define Structural Differences in Single Amino Acid Variants of Human Mitochondrial Branched-Chain Amino Acid Aminotransferase 2 (BCAT2).
Anderson LC, Håkansson M, Walse B, Nilsson CL
J Am Soc Mass Spectrom 28 1796-1804 (2017)
PMID: 28681360

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 41.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15382 (Residues: 28-392; Coverage: 93%)
Gene names: BCAT2, BCATM, BCT2, ECA40
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 84.032Å b: 84.032Å c: 105.446Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.131 0.128 0.179
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The mechanism of branched-chain amino acid transferases in different diseases: Research progress and future prospects.
Nong et al. (2022)
1 other article

3 mentions without citation

1H NMR-Based Metabolomics Coupled With Molecular Docking Reveal the Anti-Diabetic Effects and Potential Active Components of Berberis vernae on Type 2 Diabetic Rats.
Li et al. (2020)
2 more