› 5mqi

X-ray diffraction
1.85Å resolution

Crystal structure of the N-terminal domain of human Timeless

Released:
DOI: 10.2210/pdb5mqi/pdb
PDB entry 5mqi coloured by chain, front view.
PDB entry 5mqi coloured by chain, side view.
PDB entry 5mqi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the N-terminal domain of human Timeless and its interaction with Tipin.
Holzer S, Degliesposti G, Kilkenny ML, Maslen SL, Matak-Vinkovíc D, Skehel M, Pellegrini L
Nucleic Acids Res 45 5555-5563 (2017)
PMID: 28334766

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein timeless homolog Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 43.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNS1 (Residues: 1-238, 331-463; Coverage: 31%)
Gene names: TIM, TIM1, TIMELESS, TIMELESS1
Sequence domains: Timeless protein

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 68.008Å b: 71.337Å c: 185.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.197
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The TIMELESS effort for timely DNA replication and protection.
Patel et al. (2023)
6 other articles

4 mentions without citation

Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork.
Baretić et al. (2020)
3 more