› 5mtj

X-ray diffraction
1.95Å resolution

Yes1-SH2 in complex with monobody Mb(Yes_1)

Released:
DOI: 10.2210/pdb5mtj/pdb
PDB entry 5mtj coloured by chain, front view.
PDB entry 5mtj coloured by chain, side view.
PDB entry 5mtj coloured by chain, top view.
Source organisms:
Primary publication:
Selective Targeting of SH2 Domain-Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies.
Kükenshöner T, Schmit NE, Bouda E, Sha F, Pojer F, Koide A, Seeliger M, Koide S, Hantschel O
J Mol Biol 429 1364-1380 (2017)
PMID: 28347651

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166008 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Yes Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.3 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q04736 (Residues: 147-260; Coverage: 21%)
Gene names: Yes, Yes1
Sequence domains: SH2 domain
Structure domains: SH2 domain
Monobody Mb(Yes_1) Chain: B
Molecule details ›
Chain: B
Length: 98 amino acids
Theoretical weight: 10.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CXS 2 x CXS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I4122
Unit cell:
a: 101.864Å b: 101.864Å c: 139.618Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.223
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

5 review citations

Heparanase, cell signaling, and viral infections.
Koganti et al. (2020)
4 more

2 mentions without citation

Monobodies as enabling tools for structural and mechanistic biology.
Hantschel et al. (2020)
1 more