› 5mtn

X-ray diffraction
2.85Å resolution

Monobody Mb(Lck_1) bound to Lck-Sh2

Released:
DOI: 10.2210/pdb5mtn/pdb
PDB entry 5mtn coloured by chain, front view.
PDB entry 5mtn coloured by chain, side view.
PDB entry 5mtn coloured by chain, top view.
Source organisms:
Primary publication:
Selective Targeting of SH2 Domain-Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies.
Kükenshöner T, Schmit NE, Bouda E, Sha F, Pojer F, Koide A, Seeliger M, Koide S, Hantschel O
J Mol Biol 429 1364-1380 (2017)
PMID: 28347651

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138955 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Lck Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P06239 (Residues: 118-231; Coverage: 22%)
Gene name: LCK
Sequence domains: SH2 domain
Structure domains: SH2 domain
Monobody Mb(Lck_1) Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 10.05 KDa
Source organism: synthetic construct
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3221
Unit cell:
a: 91.397Å b: 91.397Å c: 88.778Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.228 0.265
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

5 review citations

Protein binders and their applications in developmental biology.
Harmansa et al. (2018)
4 more

2 mentions without citation

Monobodies as enabling tools for structural and mechanistic biology.
Hantschel et al. (2020)
1 more