› 5mto

X-ray diffraction
3.1Å resolution

N-terminal domain of the human tumor suppressor ING5 C19S mutant

Released:
DOI: 10.2210/pdb5mto/pdb
PDB entry 5mto coloured by chain, front view.
PDB entry 5mto coloured by chain, side view.
PDB entry 5mto coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Tumor Suppressor ING5 Is a Dimeric, Bivalent Recognition Molecule of the Histone H3K4me3 Mark.
Ormaza G, Rodríguez JA, Ibáñez de Opakua A, Merino N, Villate M, Gorroño I, Rábano M, Palmero I, Vilaseca M, Kypta R, Vivanco MDM, Rojas AL, Blanco FJ
J Mol Biol 431 2298-2319 (2019)
PMID: 31026448

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186799 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inhibitor of growth protein 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 12.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WYH8 (Residues: 1-105; Coverage: 44%)
Gene name: ING5
Sequence domains: Inhibitor of growth proteins N-terminal histone-binding

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P6522
Unit cell:
a: 140.94Å b: 140.94Å c: 92.323Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.254
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Understanding the interplay between CpG island-associated gene promoters and H3K4 methylation.
Hughes et al. (2020)
5 more