› 5mwb

X-ray diffraction
1.86Å resolution

Human Notch-2 EGF11-13

Released:
DOI: 10.2210/pdb5mwb/pdb
PDB entry 5mwb coloured by chain, front view.
PDB entry 5mwb coloured by chain, side view.
PDB entry 5mwb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional dissection of the interplay between lipid and Notch binding by human Notch ligands.
Suckling RJ, Korona B, Whiteman P, Chillakuri C, Holt L, Handford PA, Lea SM
EMBO J 36 2204-2215 (2017)
PMID: 28572448

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neurogenic locus notch homolog protein 2 Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 17.15 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q04721 (Residues: 414-532; Coverage: 5%)
Gene name: NOTCH2
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, XYP
Carbohydrate polymer
3 bound ligands:
Ligand FUC 1 x FUC
Ligand BGC 1 x BGC
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 20.16Å b: 49.79Å c: 125.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.264
Expression system: Drosophila melanogaster

Quick links

Citations

8 review citations

Notch signaling pathway: architecture, disease, and therapeutics.
Zhou et al. (2022)
7 more

5 mentions without citation

Structural and functional dissection of the interplay between lipid and Notch binding by human Notch ligands.
Suckling et al. (2017)
4 more