› 5mwq

Solution NMR

Biosynthetic engineered A21K-B31K-B32R human insulin monomer structure in water/acetonitrile solution

Released:
DOI: 10.2210/pdb5mwq/pdb
PDB entry 5mwq coloured by chain, ensemble of 20 models, front view.
PDB entry 5mwq coloured by chain, ensemble of 20 models, side view.
PDB entry 5mwq coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Bocian W, Kozerski L

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 32 amino acids
Theoretical weight: 3.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 25-56; Coverage: 37%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 52%
Refinement method: simulated annealing
Expression system: Escherichia coli

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