5my6

X-ray diffraction
2.25Å resolution

Crystal structure of a HER2-Nb complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-209118 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Receptor tyrosine-protein kinase erbB-2 Chain: A
Molecule details ›
Chain: A
Length: 622 amino acids
Theoretical weight: 68.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P04626 (Residues: 24-645; Coverage: 50%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Nanobody 2Rs15d Chain: B
Molecule details ›
Chain: B
Length: 115 amino acids
Theoretical weight: 12.64 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 66.52Å b: 113.52Å c: 137.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.22
Expression systems:
  • Homo sapiens
  • Escherichia coli