› 5myx

X-ray diffraction
1.49Å resolution

Structure of Pyroglutamate-Abeta-specific Fab c#24 in complex with human Abeta-pE3-18

Released:
DOI: 10.2210/pdb5myx/pdb
PDB entry 5myx coloured by chain, front view.
PDB entry 5myx coloured by chain, side view.
PDB entry 5myx coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional analyses of pyroglutamate-amyloid-β-specific antibodies as a basis for Alzheimer immunotherapy.
Piechotta A, Parthier C, Kleinschmidt M, Gnoth K, Pillot T, Lues I, Demuth HU, Schilling S, Rahfeld JU, Stubbs MT
J Biol Chem 292 12713-12724 (2017)
PMID: 28623233

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209169 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab c#24 light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 219 amino acids
Theoretical weight: 24.13 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab c#24 heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 248 amino acids
Theoretical weight: 26.56 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
P3(40) Chains: E, F
Molecule details ›
Chains: E, F
Length: 16 amino acids
Theoretical weight: 1.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 674-689; Coverage: 2%)
Gene names: A4, AD1, APP
Sequence domains: Beta-amyloid peptide (beta-APP)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 115.611Å b: 95.935Å c: 90.371Å
α: 90° β: 101.17° γ: 90°
R-values:
R R work R free
0.182 0.18 0.213
Expression systems:
  • Mus musculus
  • Not provided

Quick links

Citations

2 review citations

The Amyloid-β Oligomer Hypothesis: Beginning of the Third Decade.
Cline et al. (2018)
1 more

4 mentions without citation

Structural diversity of B-cell receptor repertoires along the B-cell differentiation axis in humans and mice.
Kovaltsuk et al. (2020)
3 more